Table 2. Kinetics of substrate oxidation by short, long and extralong MnPs from the C. subvermispora genome, two directed variants at the C-tail and 11 additional directed variants at the haem-propionate channel of the MnP short variant.
| Mn2+ | ABTS | |||||
|---|---|---|---|---|---|---|
| K m (M) | k cat (s1) | k cat/K m (s1mM 1) | K m (M) | k cat (s1) | k cat/K m (s1mM 1) | |
| Native MnPs | ||||||
| MnP13 (short) | 116 12 | 88 2 | 756 65 | 2440 200 | 93 3 | 38 2 |
| MnP5 (long) | 50 7 | 327 15 | 6600 700 | 0 | 0 | |
| MnP10 (long) | 59 9 | 331 20 | 5600 500 | 0 | 0 | |
| MnP6 (extralong) | 9 2 | 83 5 | 9540 653 | 0 | 0 | |
| MnP12 (extralong) | 11 1 | 61 0.8 | 5560 325 | 0 | 0 | |
| Short-tail variants† | ||||||
| MnP6 short variant | 16 2 | 50 1 | 3030 390 | 116 6 | 29 1 | 251 11 |
| MnP10 short variant | 34 3 | 118 4 | 3500 360 | 3430 370 | 20 1 | 5.8 0.4 |
| Propionate-channel variants‡ | ||||||
| E35L | 0 | 0 | 118 13 | 64 2 | 546 51 | |
| E39L | 0 | 0 | 250 12 | 30 1 | 120 16 | |
| G82L | 46 5 | 30 1 | 651 46 | 1290 150 | 100 4 | 77 5 |
| D85L | 107 14 | 19 1 | 179 18 | 45 8 | 4 0 | 91 15 |
| D179V | 0 | 0 | 288 12 | 76 1 | 263 13 | |
| E35L/E39L | 0 | 0 | 80 9 | 34 1 | 417 38 | |
| G82L/D85L | 0 | 0 | 0 | 0 | ||
| D85L/D179V | 0 | 0 | 35 3 | 35 1 | 977 60 | |
| E35L/E39L/G82L | 0 | 0 | 844 121 | 98 5 | 116 12 | |
| E35L/E39L/D85L | 0 | 0 | 243 17 | 14 3 | 59 3 | |
| E35L/E39L/D179V | 0 | 0 | 146 17 | 2 0 | 15 1 | |
†
Variants with an 18-residue shortened C-tail.
‡
Variants at the haem-propionate channel of the MnP6 short variant.