Table 4. Validation of microarray results by real-time quantitative PCR.

Genes	Probe Name in Microarray	Mean fold change by Microarray (Pregnant/Non-pregnant)	Mean fold change by Q-PCR (Pregnant/Non-pregnant)
CHGB	A_72_P658293	3.50*	9.17*
USP18	A_72_P417087	1.84*	3.32*
VWF	A_72_P293294	−1.85*	−3.84*
LPAR3	A_72_P406683	4.12**	6.72**
NR5A1	A_72_P444602	5.55**	12.74*
PPARD	A_72_P165296	3.47*	3.11*
BIN1	A_72_P672677	−1.69*	−3.11*

The numbers here represent the fold change of gene expression in pregnant sows versus non-pregnant sows. The numbers equal to 1.00 means the expression level doesn't change between the two groups. The numbers >1.00 means the expression level is increased in pregnant sows while numbers <−1.00 indicate that the expression level is reduced in pregnant sows. The numbers with “*” are statistically significant (P<0.05), and which with“**” are statistically very significant (P<0.01).