Table 2. Similarity, identity, and coverage of select hydrogenase proteins comparing the Rubrivivax gelatinosus CBS sensor and uptake hydrogenases to other bacterial species including Ralstonia eutropha H16.
Similarity% | Identity% | Coverage% | ||
Sensor Hydrogenase | ||||
HupU | Rx. gelatinosus IL144 | 99 | 98 | 100 |
R. eutropha H16 (HoxB) | 84 | 74 | 98 | |
HupV | Rx. gelatinosus IL144 | 99 | 98 | 100 |
R. eutropha H16 (HoxC) | 75 | 66 | 100 | |
Uptake Hydrogenase | ||||
HupA | Rx. gelatinosus IL144 | 100 | 100 | 100 |
R. eutropha H16 (HoxK) | 96 | 89 | 87 | |
HupB | Rx. gelatinosus IL144 | 99 | 99 | 100 |
R. eutropha H16 (HoxG) | 92 | 86 | 100 |
HupUV is a sensor hydrogenase, with HupU being the small subunit and HupV being the large, catalytic subunit. HupAB comprises a membrane-bound uptake hydrogenase with a small HupA subunit and a large, catalytic HupB subunit. Analysis was done using a NCBI P-BLAST search.