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. 2014 Dec 5;9(12):e114722. doi: 10.1371/journal.pone.0114722

Figure 4. Analysis of metabolite profiles of Streptomyces JCM9888 and related mutants.

Figure 4

A) HPLC-UV analysis of metabolite profiles of Streptomyces: i) Wild type JCM9888, ii) ΔacmG mutant, iii) ΔacmK mutant, iv) ΔacmE mutant. The products DACM (1), ACM (2) are indicated. B) HPLC-ESI-MS analysis of metabolite profile of JCM9888 under the same program of HPLC-UV. C) The molecular formula of compounds DACM (1), ACM (2) were determined to be C10H13ClN6O6S (m/z  = 381.0384, 383.0354 [M+H]+) and C13H18ClN7O7S (m/z  = 452.0755, 454.0725 [M+H]+) on the basis of ESI-HRMS. D) MS/MS pattern of ascamycin and dealanylascamycin are shown and the detected data is closely consistent with anticipated fragmentation ions. D1: dealanylascamycin; D2: ascamycin.