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Gating strategy for the analysis of B-cell subsets. Lymphocytes were gated according to forward and side scatter (A). B-cells were identified as CD19-expressing cells in the lymphocyte population (B). A CD27/CD19 dot plot defined total CD27+ CD19+ memory B-cells (C). The double staining of B-lymphocytes for CD27 and IgD made it possible to determine the percentages of naive B-cells (IgD+ CD27−), switched memory B-cells (IgD− CD27+) and non-switched memory B-cells (IgD+ CD27+) (D).
