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Changes in the percentage and absolute number of memory B-cells subsets with age. The proportions of non-switched memory B-cells (CD27+ IgD+) (A) and switched memory B-cells (CD27+ IgD−) (C) among CD19+ lymphocytes were analyzed by flow cytometry on whole-blood samples. The corresponding absolute numbers (B and D) were calculated from the absolute number of B-cells. Solid horizontal lines indicate the median values for each age group. m = month; y = year.
