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. Author manuscript; available in PMC: 2014 Dec 5.
Published in final edited form as: Nat Struct Mol Biol. 2014 Apr 20;21(5):464–471. doi: 10.1038/nsmb.2815

Figure 3. Genetic evidence for base-triple interactions between the AGC triad and the ACAGAGA region of the U6 snRNA.

Figure 3

(a,b) Spot assays showing growth on selective media of equivalent numbers of yeast cells containing combinations of alleles at G52 and G60 (a) or C61 (b). (c,d) Spot assays showing growth on selective media of equivalent numbers of yeast cells containing combinations of alleles at A53 and A59 (c) or C61 (d). Matrices are presented as in Figs. 2a,b. Note that in a yeast also contained a mutation at position 59 in U4 to repair U4/U6 stem I (ref. 19). For additional positional specificity controls see Supplementary Fig. 1. (e,f) Diagrams of observed (group II intron) and predicted (spliceosome) isomorphic base-triple interactions11 involving the first (f) and second (e) residues of the catalytic triad. Diagrams are as in Fig. 2c.