Table 3. Control studies of VPSPr inoculations to Tg(HuPrP)*.
| Inoculum | Tg(HuPrP) | No. with clinical signs/total | Histology, immunohistochemistry |
PrPSc Western blot |
|||
|---|---|---|---|---|---|---|---|
| No. positive/total | Dpi positive/total† | No. positive/total | Dpi positive/total | ||||
| Negative controls | |||||||
| None | (129V)×8‡ |
0/15 | 0/9 | 0/721 ± 43 | 0/4 | 0/704 ± 43 | |
| Noninfectious |
0/17 |
0/8 |
0/744 ± 15 |
|
0/5 |
0/749 ± 112 |
|
| Positive controls | |||||||
| sCJDVV2§ | (129V)×8 | 6/6 | 3/3 | 223 ± 13/223 ± 13 | 6/6 | 223 ± 11/223 ± 11 | |
| sCJDVV1 | 7/7 | 7/7 | 353 ± 12/353 ± 12 | 7/7 | 353 ± 12/353 ± 12 | ||
| sCJDMM1 | (129M)×2 | 3/3 | 3/3 | 183 ± 22/183 ± 22 | 3/3 | 183 ± 22/183 ± 22 | |
| sCJDMM2 | 3/3 | 3/3 | 609 ± 139/609 ± 139 | 3/3 | 609 ± 139/609 ± 139 | ||
| sCJDMV2 | (129V)×3 | 6/6 | 6/6 | 312 ± 4/312 ± 4 | NA | NA | |
Dpi, days postinoculation; M, methionine; V, valine; NA, not available; VPSPr, variably protease-sensitive prionopathy; sCJD sporadic Creutzfeldt-Jakob disease. †Dpi of positive mice compared with dpi of all mice (including positive mice). Each value represents the mean ± SD. ‡Refers to the presence of V or M at PrP residue 129 and expression level indicated as × normal. §Identifies the sCJD case-patients providing the inoculum.