Table 4. VPSPr inoculations to Tg(HuPrP): second passage*.

Inoculum	Tg(HuPrP)	No. with clinical signs/total	Histology, immunohistochemistry			PrPSc Western blot
			No. positive/total	Dpi positive/total†		No. positive/total	Dpi positive/total
Tg(HuPrP129V) inoculated with VPSPr-129VV 2nd‡	(129V)×8§	0/10	0/10	0/616 ± 135		0/10	0/616 ± 135
Tg(HuPrP129M) inoculated with VPSPr-129MM 1st	(129M)×2	0/3	0/3	0/694 ± 51		NA	NA

Dpi, days postinoculation; M, methionine; V, valine; NA, not available; VPSPr, variably protease-sensitive prionopathy associated with homozygosity V or M at codon 129 of the PrP gene (VPSPr-129VV or VPSPr-129MM);
†Dpi of positive mice compared with dpi of total mice (including the positive mice). Each value represents the mean ± SD.
‡Identifies the Transgenic mice, inoculated with VPSPr in the first passage, providing the inoculum.
§Refers to the presence of V or M at PrP residue 129 and expression level indicated as × normal.