Table. Primers and probe used to detect the BTV strain BTV-n in samples from goats, Corsica, France, 2014*.
| PCR type | Target | Probe and primer names | Primer sequence, 5′ → 3′ | Nucleotide location |
|---|---|---|---|---|
| Conventional BTV RT-PCR | Segment 2 | PF | YRWTTGATTTTGARAARGA | 1549–1566 |
|
|
|
PR |
GAAYCGACCACTGCCTATG |
2355–2337 |
| Conventional RT-PCR | BTV-n segment 2 | BTV-n F | CAGATCTGGTTTTACCGAG | 1546–1564 |
|
|
|
BTV-n R |
ATGATCCATCGGACTTAACT |
1949–1927 |
| Real-time RT-PCR | BTV-n segment 2 | BTV-n F3 | TGGATCATGATGGTTATGAACACC | 1942–1966 |
| BTV-n R3 | CGCCTCTCCAATCTCACGTATT | 2102–2081 | ||
| BTV-n | FAM-TGACTATGCGAGGTTGG-MGB | 1987–2004 |
*BTV, bluetongue virus; RT, reverse transcription; PF, forward primer; PR, reverse primer.