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. 2014 Dec;20(12):2123–2125. doi: 10.3201/eid2012.140924

Table. Primers and probe used to detect the BTV strain BTV-n in samples from goats, Corsica, France, 2014*.

PCR type Target Probe and primer names Primer sequence, 5′ → 3′ Nucleotide location
Conventional BTV RT-PCR Segment 2 PF YRWTTGATTTTGARAARGA 1549–1566


PR
GAAYCGACCACTGCCTATG
2355–2337
Conventional RT-PCR BTV-n segment 2 BTV-n F CAGATCTGGTTTTACCGAG 1546–1564


BTV-n R
ATGATCCATCGGACTTAACT
1949–1927
Real-time RT-PCR BTV-n segment 2 BTV-n F3 TGGATCATGATGGTTATGAACACC 1942–1966
BTV-n R3 CGCCTCTCCAATCTCACGTATT 2102–2081
BTV-n FAM-TGACTATGCGAGGTTGG-MGB 1987–2004

*BTV, bluetongue virus; RT, reverse transcription; PF, forward primer; PR, reverse primer.