Table 1. GOLV and HEBV screening results for dead pigs on 1 farm in Gyeonggi Province, Republic of Korea, 2013*.
Pig no. | Clinical signs | Age group† | Month sample collected | Sample no. | Sample type | GOLV |
HEBV |
Other pathogens | |||
---|---|---|---|---|---|---|---|---|---|---|---|
RT-PCR | qRT-PCR, copies/μL‡ | RT-PCR | qRT-PCR, copies/μL‡ | ||||||||
1 | None | Finisher | Mar | P0 | Lung | – | NA | + | 2.57 × 103 | ||
2 | Wasting, cyanosis, fever, respiratory disorders, diarrhea | Weaned | Mar | P1 | Lung§ | +¶ | 2.03 × 103 | +# | 1.26 × 102 | NA | |
P2 | Intestine | + | 1.27 × 104 | + | 1.16 × 102 | NA | |||||
3 | Diarrhea, respiratory disorders | Finisher | Mar | P3 | Lung | + | 1.11 × 102 | + | 1.37 × 103 | PRRSV, PCV2 | |
P4 | Intestine | – | NA | – | . | ||||||
4 | Diarrhea, respiratory disorders | Gilt | Mar | P5 | Lung | + | 2.53 × 103 | + | 5.45 × 104 | PRRSV, PCV2 | |
P6 | Intestine | – | NA | – | NA | Rotavirus, E. coli | |||||
5 | Respiratory disorders | Weaned | Apr | P7 | Lung | + | 2.04 × 103 | + | 2.78 × 103 | PRRSV, PCV2 | |
P8 | Intestine | +¶ | 1.82 × 102 | – | NA | ||||||
6 | Diarrhea, respiratory disorders | Grower | Apr | P9 | Lung | + | 5.10 ×1 05 | +# | 5.46 × 103 | PRRSV | |
P10 | Intestine | – | NA | – | NA | ||||||
7 | Diarrhea | Finisher | Mar | P11 | Intestine | – | NA | – | NA | E. coli | |
8 | Diarrhea | Sow | Mar | P12 | Intestine | + | 6.52 × 102 | – | NA | Rotavirus, E. coli | |
9 | None | Finisher | Mar | P13 | Intestine | – | NA | – | NA | NA |
*E. coli, Escherichia coli; GOLV, Gouleako virus; HEBV, Herbert virus; NA, not applicable; qRT-PCR, quantitative reverse transcription PCR; PCV2, porcine circovirus type 2; PRRSV, porcine reproductive and respiratory syndrome virus, Rota, rotavirus. †Samples were sorted into 6 groups: female (gilt and sow), suckling (<30 d), weaned (30–60 d), grower (60–90 d); and finisher (≥90 d). ‡Amount of virus from qRT-PCR results, copies/μL (online Technical Appendix, http://wwwnc.cdc.gov/EID/article/20/12/13-1742-Techapp1.pdf). §First sample in which virus was detected by particle-associated nucleic acid–-random PCR. ¶GenBank, GOLV (P1, KF361520; P8, KF361521). #GenBank, HEBV (P1, KF361522; P9, KF362523).