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. Author manuscript; available in PMC: 2015 Jun 1.
Published in final edited form as: Gene Ther. 2014 Sep 18;21(12):1029–1040. doi: 10.1038/gt.2014.82

Fig. 1. Widespread transduction of the dorsal hippocampus following AAV8 and AAV-mediated gene transfer.

Fig. 1

(a) Representative images showing the rostral-caudal distribution of GFP immunoreactivity in the hippocampus following AAV8 and AAV9 vector infusion. The rostral-caudal coordinate (in mm) for each section relative to bregma is shown. (b) Transduction volumes (mm3) in the hippocampus. Bars represent the mean + SEM, n = 3–4. Unpaired t-test, P = 0.08 (c) GFP immunoreactivity in the hilar region, CA3 and CA1 regions in representative AAV8 and AAV9-injected brains (upper dentate granule cell layer (DF), mossy fibre pathway (MF), corpus callosum (CC) are shown as reference points, with the inset showing a higher magnification view of GFP-positive CA1 neurons. (d) Double-immunofluorescent GFP labeling with GFAP, NeuN and Iba1 showing co-localisation of GFP signal in both astrocytes and some neurons (arrowheads) with both vector serotypes but not microglia. Scale bar, (a) 1 mm, (c) 250 um, (d) 80 um