Figure 1. Induction of c-fos-ZsYellow1 in vitro.
A. Quantification of fluorescence from SH-SY5Y cells transiently transfected with pLenti-6-c-fos-ZsYellow1-V5, and treated with TPA (closed circles) or control DMSO (empty circles). Average with SEM represents the number of fluorescent cells counted from three independent experiments. B. SH-SY5Y cells 30 minutes before and (C) one hour after activation with TPA. Scale bars correspond to 20 μm.