Skip to main content
. Author manuscript; available in PMC: 2015 Jun 1.
Published in final edited form as: Nat Med. 2014 Nov 17;20(12):1444–1451. doi: 10.1038/nm.3717

Figure 4.

Figure 4

MiR-124 targets AMPAR subunits, and its expression is reduced in the cortex of tTA:CHMP2BIntron5 mice. (a) Alignment of potential binding sites for miR-124 in the 3′UTRs of Gria2, Gria3, and Gria4 mRNAs. (b) Direct interaction between 3′UTRs of Gria2, Gria3, and Gria4 mRNAs and miR-124 (n > 4 assays, *: P < 0.05, ***: P < 0.001 by two-sided t test). (c) The effects of miR-124 on the expression of luciferase-containing subunit 3′UTRs in which we mutated the miR-124 binding site. n = 4 assays, *: P < 0.05, **: P < 0.001 by two-sided t test. (d) Northern blot analysis of miR-9 and miR-124 expression levels in the cortex of tTA:CHMP2BWT and tTA:CHMP2BIntron5 mice at different ages. (e) Quantification of the northern blots in panel d (n = 3 mice per group; *: P < 0.05, **: P < 0.01 by two-sided t test). (f) Fluorescence in situ hybridization of miR-124 in brain sections of tTA:CHMP2BWT and tTA:CHMP2BIntron5 mice at 8 months of age. (g) Double-fluorescence in situ hybridization staining of miR-124 (red) in CHMP2BIntron5-expressing cells (green) in the cortex at 8 months of age. Scale bar, 150 μm in f and g. (h) Higher magnification of the boxed area in G. Scar bar, 20 μm. Values are mean ± s.e.m. in b and c and mean ± s.d. in e.