Table 3.
High‐performance liquid chromatography measurements for markers of the serotonin and catecholamine systems in the occipital cortex in the fetal baboon model of prenatal nicotine exposure
| Measure | Control mean ± SEM (range) (n = 5) | Nicotine‐exposed mean ± SEM (range) (n = 5) | FDR‐corrected P‐value |
|---|---|---|---|
| 5‐HT (pmol/mg protein) | 4.33 ± 0.59 (3.2, 6.1) | 3.65 ± 0.40 (2.9, 5.1) | 0.40† |
| 5‐HIAA (pmol/mg protein) | 11.06 ± 1.45 (8.2, 16.6) | 14.04 ± 1.23 (10.7, 17.0) | 0.35 |
| Norepinephrine (pmol/mg protein) | 5.53 ± 2.63 (0, 15.1) | 1.81 ± 0.50 (0.5, 3.4) | 0.42† |
| Dopamine (pmol/mg protein) | 3.25 ± 0.45 (1.9, 4.5) | 1.81 ± 0.14 (1.3, 2.1) | 0.21 |
| DOPAC (pmol/mg protein) | 36.88 ± 7.59 (18.6, 64.5) | 34.76 ± 7.67 (21.6, 64.1) | 0.71† |
| 5‐HIAA : 5‐HT ratio | 2.62 ± 0.24 (1.9, 3.2) | 4.11 ± 0.65 (2.1, 5.3) | 0.24 |
| DOPAC : dopamine ratio | 12.26 ± 2.70 (4.9, 19.2) | 21.17 ± 7.11 (11.2, 49.3) | 0.50† |
† Wilcoxon rank–sum used instead of t‐test (see Materials and Methods).
5‐HIAA = 5‐hydroxyindoleacetic acid; 5‐HT = 5‐hydroxytryptamine; DOPAC = 3,4‐dihydroxyphenylacetic acid; FDR = false discovery rate; SEM = standard error of the mean.