Fig. 4.

Phenotypic analysis of brachyury native and agonist peptide-generated T-cell lines. T-cell lines were generated with brachyury native and agonist peptides from the same patient #2 with prostate cancer and were designated T(native) and T(agonist), respectively. At IVS 5 T(native) was stimulated with either native or agonist peptide, and T(agonist) was stimulated with agonist peptide. On day 5 after stimulation, phenotypic analysis by flow cytometry was performed. The experiment was repeated three times, and representative FACS plots are shown. For the first two rows, cells were first gated on CD8⁺ and the percent positive out of the CD8 gate is shown in each plot. For the third row, cells were first gated on CD8⁺/Ki67⁺/CCR7^neg and the percent positive shown is of total CD8