Figure 5.

Stationary S. cerevisiae cells grown in biofilms and planktonic cultures have similar drug tolerance. Cultures were grown for 48 hours and viability was measured as CFUs. Cells were treated with 10 times MIC of the indicated antifungal drug, or left untreated as a control, and CFUs were measured after 24 hours. Shown is log change in viability. White bar: wild type S. cerevisiae Σ1278b grown as biofilms on polystyrene surfaces. Grey bar: wild type grown planktonically in glass flasks. Black bar: flo11 knockout mutant grown planktonically in glass flasks. VOR: voriconazole (10 μg/ml), 5FC: flucytosine (80 μg/ml), CAS: caspofungin (10 μg/ml), AmB: amphotericin B (10 μg/ml). n = 3, error bars show standard deviation. Statistical significance between treated and untreated samples was evaluated with Student’s t-test. *P < 0.01.