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. 2014 Nov 26;13:254. doi: 10.1186/1476-4598-13-254

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© Chen et al.; licensee BioMed Central Ltd. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Expression of epithelial and mesenchymal protein markers are regulated by SIRT1 in HOK and OSCC cells. (A) Western blotting revealed the expression levels of epithelial and mesenchymal protein markers in HOK and OSCC cell lines. Equal amounts of cell protein (20 ug) were immunoblotted with antibodies against SIRT1, E-cadherin, vimentin, N-cadherin, and β-actin. (B) Loss of SIRT1 increased expression of endogenous mesenchymal protein markers. Western blotting revealed expression of SIRT1 and EMT markers in HOK cells with or without siSIRT1. (C) Ectopic expression of SIRT1 increased expression of E-cadherin and reduced expression of vimentin and N-cadherin. Equal amounts of protein (20 ug) from OECM1 cells were transient transfected with pEGFP-SIRT1 or vector alone (pEGFP-C1) and analyzed by Western blot.