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. 2014 Nov 26;13:254. doi: 10.1186/1476-4598-13-254

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© Chen et al.; licensee BioMed Central Ltd. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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SIRT1 down-regulates expression and activity of MMP7. (A) qRT-PCR revealed the expression levels of MMP7 in OSCC cell lines after transient transfection with pEGFP-SIRT1 or vector alone (pEGFP-C1). Each data point represents the mean ± SD from at least three independent experiments. The asterisk indicates statistically significant difference (*, p <0.05) comparing to the control. (B) The MMP7 protein level was assessed by immunoblotting with anti-MMP7 antibody and GAPDH. MMP7 proteins in the cell homogenates were evaluated. (C) MMP7 activity was assayed by casein zymography. MMP7 activities in the cell media were compared between SIRT1-overexpressing or silencing OECM1 cells. (D) MMP7 concentrations and protein levels in OSCC cell media were assessed by ELISA and immunoblotting with anti-MMP7 antibody and GAPDH. Cell culture media were collected and concentrated from SIRT1-overexpressing or silencing OSCC cell lines.