. 2014 Oct 30;45(1):107. doi: 10.1186/s13567-014-0107-6

Table 1.

Vaccination schemes of the four studies

Study	Chickens	1 ^st vaccination at day 0		2 ^nd vaccination at day 21 (studies 1 and 2) or at day 14 (studies 3 and 4)		Challenge
Study	Chickens	Vaccine	Dose	Vaccine	Dose	Challenge
1	SPF	vFP2211	10³ TCID₅₀	-	-	No
	H5N9 + FP MDA	vFP2211	10³ TCID₅₀	-	-
		vFP2211	10⁵ TCID₅₀	-	-
		vFP2211	10⁵ TCID₅₀	inH5N9	0.5 mL
		-	-	inH5N9	0.5 mL
2	H5N9 + FP MDA	inH5N9	0.2 ml	inH5N9	0.5 mL	No
	H5N9 + FP MDA	vFP89	10³ TCID₅₀	inH5N9	0.5 mL
	SPF	vFP89	10³ TCID₅₀	inH5N9	0.5 mL
3	SPF	vFP89	10³ TCID₅₀	inH5N9	0.3 mL	No
	SPF	-	-	inH5N9	0.3 mL
	H5N9 MDA	vFP89	10³ TCID₅₀	inH5N9	0.3 mL
	H5N9 MDA	-	-	inH5N9	0.3 mL
	FP	vFP89	10³ TCID₅₀	inH5N9	0.3 mL
	FP	-	-	inH5N9	0.3 mL
	H5(N8)* + H5N9 + FP MDA	vFP89	10³ TCID₅₀	inH5N9	0.3 mL
4	SPF	-	-	-	-	Yes (D28)
	SPF	vFP89	10³ TCID₅₀	-	-
	FP MDA	vFP89	10³ TCID₅₀	inH5N9	0.3 mL
	FP MDA	-	-	inH5N9	0.3 mL
	H5N9 MDA	-	-	-	-
		vFP89	10³ TCID₅₀	-	-
		vFP89	10³ TCID₅₀	inH5N9	0.3 mL
		-	-	inH5N9	0.3 mL

Chickens were hatched from unvaccinated (SPF) or vaccinated (MDA) hens. Chicks with H5N9, FP, H5N8 and/or H5N1 MDA were the progeny of hens vaccinated with inH5N9, fowlpox (FP), vFP89 (HA gene from A/turkey/Ireland/1378/83 [H5N8]) and/or vFP2211 (HA gene from A/chicken/Indonesia/7/2003 [H5N1]), respectively. The groups of 9 to 10 one-day-old birds received either vFP2211 or vFP89 or inH5N9 at D0, followed by a boost of inH5N9 at D21 (studies 1and 2) or at D14 (studies 3 and 4). In addition, a HPAI H5N1 challenge was performed in study 4.

*H5(N8) MDA are directed against the HA only of A/turkey/Ireland/1378/83 [H5N8]. Breeders of these chicks were indeed vaccinated with a fowlpox expressing the HA of H5N8 (vFP89).