Table 3.
H5N1 and H5N9 HI titers in breeders and their progeny
| Study | Type of breeders (age at egg harvest) | HI titers* in breeders | HI titers in progeny | |||
|---|---|---|---|---|---|---|
| Age at blood sampling | H5N1 | H5N9 | H5N1 | H5N9 | ||
| 1 | H5N9 + FP (42 weeks) | 36 weeks | 4.0 ± 1.8 | 7.5 ± 2.2 | 2.0 ± 2.2 | 3.5 ± 3.0 |
| 44 weeks | 2.9 ± 2.1 | 7.5 ± 2.5 | ||||
| 2 | H5N9 + FP (62 weeks) | 60 weeks | 3.5 ± 1.9 | 6.5 ± 2.7 | 1.0 ± 1.7 | 3.1 ± 2.7 |
| 68 weeks | 3.0 ± 1.9 | 6.6 ± 2.1 | ||||
| 3 | H5N9 (24-25 weeks) | 20 weeks | 4.9 ± 2.0 | 7.1 ± 1.7 | 3.0 ± 1.9 | 4.3 ± 2.0 |
| 28 weeks | 4.3 ± 2.5 | 5.9 ± 2.0 | ||||
| H5(N8) + H5N9 + FP (24-25 weeks) | 20 weeks | ND** | 7.0 ± 1.6 | 1.8 ± 1.1 | 3.0 ± 1.9 | |
| 28 weeks | ND | 5.3 ± 1.9 | ||||
| 4 | H5N9 (58 weeks) | 52 weeks | 3.9 ± 2.7 | 5.0 ± 2.1 | ND | 3.4 ± 0.9 |
| 60 weeks | 4.6 ± 2.2 | 5.8 ± 2.3 | ||||
Breeders hens were vaccinated with inH5N9 and fowlpox (H5N9 + FP), inH5N9 only (H5N9) or with vFP89, inH5N9 and fowlpox (H5(N8) + H5N9 + FP). Their serums were taken at two time points flanking the time of harvest of their eggs. Serums of their progeny were sampled at 1 day-of-age. NIBRG14 and NIRG23 H5N1 antigens were used in studies 1 & 2 and studies 3 & 4, respectively.
*mean HI titer ± standard deviation in log2.
**ND means not done.