Table 4.
Results of the vaccination-challenge study (Study 4)
| Group | MDA | Vaccine | H5N8 HI titers a | H5N9 HI titers | Protection b | Oropharyngeal shedding c | |||
|---|---|---|---|---|---|---|---|---|---|
| D0 | D14 | D14 | D28 | D28 | (MDT) | 2 Dpc | 4 Dpc | ||
| 1 | - | - | - | NDd | 0/10 (2.0 ± 0.0) | 0/10 (2.0 ± 0.0) | 0/10 (2.0) | 10/10 (7.8 ± 0.3) | ND |
| 2 | - | vFP89 | - | 10/10 (3.7 ± 0.8) | 10/10 (5.5 ± 1.2) | 8/10 (2.9 ± 0.6) | 1/10 (3.4) | 10/10 (6.3 ± 1.3) | 5/5 (6.6 ± 0.7) |
| 3 | FP | vFP89 | inH5N9 | 2/9 (2.3 ± 0.7) | 9/9 (8.1 ± 1.6) | 9/9 (7.6 ± 1.8) | 9/9 | 6/9 (4.6 ± 0.7) | 4/9 (4.0 ± 0.5) |
| 4 | FP | - | inH5N9 | ND | 9/9 (5.1 ± 1.6) | 9/9 (5.8 ± 0.7) | 5/9 (7.3) | 7/9 (4.9 ± 0.9) | 4/9 (4.6 ± 1.0) |
| 5 | H5N9 | - | - | ND | 0/9 (2.0 ± 0.0) | 0/9 (2.0 ± 0.0) | 0/9 (2.0) | 9/9 (7.3 ± 0.1) | ND |
| 6 | H5N9 | vFP89 | - | ND | 5/9 (2.9 ± 0.9) | 0/9 (2.0 ± 0.0) | 0/9 (2.7) | 9/9 (7.1 ± 0.5) | 1/1 (7.7) |
| 7 | H5N9 | vFP89 | inH5N9 | 0/9 (2.0 ± 0.0) | 9/9 (5.8 ± 1.0) | 9/9 (4.3 ± 1.1) | 7/9 (7.0) | 6/9 (4.5 ± 0.6) | 7/9 (4.6 ± 1.3) |
| 8 | H5N9 | - | inH5N9 | 0/9 (2.0 ± 0.0) | 4/9 (3.2 ± 1.6) | 6/9 (3.7 ± 1.7) | 4/9 (5.0) | 8/9 (5.0 ± 1.0) | 7/8 (5.3 ± 1.1) |
Results of serology, protection and oropharyngeal viral shedding in chicks with or without FP or H5N9 MDA vaccinated or not with vFP89 and/or inH5N9 and challenged at D28 with an HPAI H5N1 Indonesian isolate (A/chicken/West Java-Subang/29/2007).
aNumber of positive birds (HI titer ≥ 3 log2)/total; the mean HI titer ± standard deviation is given between brackets.
bNumber of birds protected against mortality/total; the mean death time (MDT) is given between brackets.
cNumber of positive birds (≥3.8 log10 EID50-equivalent)/total; the mean viral RNA load ± standard deviation is given in log10 EID50-equivalent between brackets; Dpc, day post-challenge.
dND means not done.