Fig 5.

AnxA1 regulates macrophage functions through stimulation of IL-10 production. Hepatic macrophages were isolated from livers of either control or 8-week MCD-fed mice. (A) F4/80-positive cells were analyzed by FCM for the coexpression of AnxA1 and IL-10 or MCD-fed mice. (B) Macrophages isolated from livers of MCD-fed mice were incubated in vitro with or without recombinant AnxA1 (100 nM) and the p38MAPK inhibitor, SB203880 (SB; 10 μM), and subsequently analyzed for expression of IL-10 mRNA (B). (C and D) RT-PCR analyses for other markers, including M1 (iNOS and IL-12p40) and (F and G) M2 activation markers (arginase-1 and MGL-1/CD301), as well as (E and H) CCL2 or CCR2. Values refer to four to five different cell preparations and are presented as mean ± standard deviation. Statistical differences were assessed by one-way ANOVA test with Tukey's correction for multiple comparisons.