Fig 7.

AnxA1 regulates galectin-3 production in livers of mice with NASH. WT and AnxA1 KO C57BL/6 mice were fed the MCD diet for 8 weeks. (A and B) Galectin-3 mRNA (boxes and whiskers) and protein levels (mean ± standard deviation) were measured by RT-PCR and western blotting, respectively. Data are from 6-8 mice per group and statistical differences were assessed by one-way ANOVA test with Tukey's correction for multiple comparisons. (C) Macrophages isolated from livers of control (Cont) or 4-week MCD-fed mice were incubated with or without recombinant AnxA1 (100 nM) and the p38MAPK inhibitor, SB203880 (SB; 10 μM), for 16 hours before quantification of galectin-3 mRNA. (D and E) Liver galectin-3 expression by IHC in MCD-fed animals (magnification, 400×). Enlargement shows cell foci containing galectin-3 macrophages and HSCs.