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. Author manuscript; available in PMC: 2015 Dec 5.
Published in final edited form as: Circ Res. 2014 Oct 6;115(12):997–1006. doi: 10.1161/CIRCRESAHA.115.304591

Figure 2. TYMP deficiency attenuates platelet activation in vitro.

Figure 2

A & B. Platelet-rich plasma isolated from Tymp−/−, Tymp+/− or WT mice was used for platelet aggregation assay induced by (A) 2.5 μM ADP, and (B) 1 μg/ml collagen using a standard turbidimetric assay. N=6. C & D. Washed murine platelets were used for aggregation assay induced by (C) 0.05 U/ml thrombin or (D) 0.5 μg/ml collage-related peptide (CRP) (n=4 or 5). E. Platelets in platelet-rich plasma (20 μl) were mixed with Tyrode’s buffer (80 μl), and stimulated with indicated agonists for 5 min at room temperature. Reactions were stopped by adding 2% formaldehyde, 1 mM EDTA in PBS. P-select in expression was stained by FITC-conjugated antibody and examined by flow cytometry. *p<0.05 WT vs. Tymp+/− and Tymp−/−; # P<0.05, WT vs. Tymp−/−, N=3.