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. Author manuscript; available in PMC: 2015 Dec 5.
Published in final edited form as: Circ Res. 2014 Oct 6;115(12):997–1006. doi: 10.1161/CIRCRESAHA.115.304591

Figure 4. TYMP interacts with Lyn and discriminately regulates phosphorylation of Lyn, PECAM-1 (CD31) and AKT in platelets.

Figure 4

A. Human platelet lysates were used for immunoprecipitation of Lyn and then immunoblotting was performed for TYMP and Lyn. Blots represent at least 3 independent experiments. *p<0.05 vs. corresponding times of KIN59 treatments. B. Human platelets were pretreated with vehicle or KIN59 and then stimulated with collagen for indicated times. Platelets lysates were analyzed by immunoprecipitation and immunoblotting assays using indicated antibodies. Bar graph shows Lyn activity from 3 independent experiments. *p<0.05 vs. corresponding times of KIN59 treatments. C. Human platelets treated as in panel A were analyzed by Western blot using indicated antibodies. Bar graph represents 3 independent experiments. D. Mouse platelets pooled from 10–12 mice were divided into 3 groups and stimulated with CRP as indicated. Platelet lysates were used for immunoprecipitation and immunoblotting assays using indicated antibodies. Bar graph shows the ratio of phosphorylated AKT and PECAM1 to their total proteins.