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. Author manuscript; available in PMC: 2016 Jan 1.
Published in final edited form as: J Immunother. 2015 Jan;38(1):1–11. doi: 10.1097/CJI.0000000000000062

Figure 4. Combinatorial treatment increases the percentage of IFNγ secreting CD8+ T cells and tumor specific CD8+ T cells in the tumor microenvironment.

Figure 4

CD8+ T cells were isolated and purified from spleen and livers on day 13 after hemispleen implantation of Panc02 tumor cells. Tumor-bearing mice were treated with Cy, GVAX or αPD-1/αPD-L1 therapy as indicated. The percentage of IFNγ+ producing CD8+ T cells amongst all CD8+ T cells in (A) splenocytes and (B) tumor infiltrating lymphocytes is shown. ELISA assays were performed using autologous irradiated Panc02 tumor cells as antigenic targets for CD8+ T cells isolated from (C) spleen and (D) tumor infiltrating lymphocytes. Each experimental group consisted of five mice, pooled and analyzed individually in triplicates. Data represent mean ± SEM from one representative experiment that was repeated at least twice. *p<0.05, ** p<0,01, *** p< 0.001, TIL tumor-infiltrating lymphocytes