Skip to main content
. Author manuscript; available in PMC: 2015 Dec 1.
Published in final edited form as: Clin Exp Metastasis. 2014 Oct 31;31(8):945–959. doi: 10.1007/s10585-014-9682-1

Figure 3. Mutation of transcription factor binding sites in the Gli2 promoter blocks Wnt signaling- mediated Gli2 transcription.

Figure 3

MDA-MB-231 cells co-transfected with β-catenin/TCF4/Smad3, β-catenin/TCF4, Smad3, or pcDNA control with (A) mutant Smad (mSmad) or (B) mutant TCF (mTCF) Gli2 promoter luciferase-reporter construct (normalized to Renilla and pGL3 luciferase values). n=3 biological replicates. Graphs represent mean + standard error of the mean, and p-values determined by 1-way ANOVA with Tukey Kramer analysis of means, where *p<0.05 and **p<0.01 versus pcDNA control.