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. Author manuscript; available in PMC: 2015 Dec 1.
Published in final edited form as: Clin Exp Metastasis. 2014 Oct 31;31(8):945–959. doi: 10.1007/s10585-014-9682-1

Figure 4. Rigidity activates Wnt signaling in breast cancer cells and induces nuclear β-catenin accumulation.

Figure 4

MDA-MB-231 cells transfected with TOPFlash luciferase-reporter construct and seeded onto tissue culture polysterene (TCPS) without fibronectin (Fbn), TCPS+Fbn, or polyacrylamide (PA) gel substrates for (A) 4 hours and (B) 24 hours. (C) Western blot for β-catenin accumulation in cytoplasmic and nuclear fractions of MDA-MB-231 cells seeded onto TCPS, TCPS+Fbn, or PA gel for 24 hours. n=3 biological replicates and Western blot representative of 3 biological replicates. Graphs represent mean + standard error of the mean, and p-values determined by 1-way ANOVA with Tukey Kramer analysis of means, where ***p<0.001 and ****p<0.0001.