Table 1.
Wnt signaling PCR-based microarray.
| Gene family | Gene name | PCR micro-array | SYBR green PCR validation | SYBR Catolog # |
|---|---|---|---|---|
| Canonical Wnt signaling | DIXDC1 | +5.3 | +3.1 | QT00019481 |
| FZD8 | +4.3 | +4.3 | QT00212128 | |
| DVL1 | +3 | +1.7 | QT00012663 | |
| FZD3 | +2.8 | +2 | QT00009114 | |
| FZD4 | +2.7 | +1.9 | QT00200984 | |
| FZD6 | +1.9 | +1.1 | QT00047670 | |
| FZD7 | −2.3 | −1.3 | QT00213850 | |
| AXIN1 | −1.2 | −1.8 | QT00076202 | |
| DVL2 | −1.2 | −2.9 | QT00019810 | |
| Protein ubiquitination | BTRC (β-TrCP) | +11.1 | +2.5 | QT00080787 |
| FBXW2 | +8.9 | +2.3 | QT00078225 | |
| FBXW11 | +3.3 | +2.3 | QT00083251 | |
| FBXW4 | +1.5 | +2.9 | QT00088123 | |
| Non-canonical Wnt signaling | WNT5B | +8.9 | nd | QT00033558 |
| WNT5A | +2.4 | +4 | QT00025109 | |
| WNT16 | +2.2 | nd | QT00048447 | |
| WNT4 | −2.5 | nd | QT00041391 | |
| WNT10A | −1.6 | +1.5 | QT00205632 | |
| WNT3 | −1.4 | −2.9 | QT00222698 |
MDA-MB-231 cells were seeded onto a stiff substrate (TCPS+Fbn) or soft substrate (PA Gel) and harvested for use in a Wnt signaling PCR-based microarray. Genes that were increased (plus sign and bold font) or decreased (minus sign) more than 1.2-fold on TCPS+Fbn versus PA Gel are presented above. Data are presented as fold-change differences in mRNA levels. PCR micro-array data are in the left column and PCR validation with SYBR green primers is in the right column. The far right column lists the catalog numbers for the commercial SYBR primers used to validate the PCR micro-array. n=3 biological replicates for micro-array and for SYBR green validation.