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. Author manuscript; available in PMC: 2015 Dec 4.
Published in final edited form as: Cell. 2014 Dec 4;159(6):1300–1311. doi: 10.1016/j.cell.2014.11.017

Figure 4. Quantification of PG synthesis and turnover following beta-lactam treatment.

Figure 4

Cells of TU278 [ΔlysA ΔampD] or its Δslt derivative (HC419) were grown, radiolabeled, and analyzed as in Figure 3. The amount of UDP-MurNAc-pentapeptide and total anhydromuropeptides produced were quantified from the area under the peaks in HPLC chromatograms. Radiolabel incorporation into PG was determined by quantifying the amount of label released from cells by lysozyme. Antibiotic concentrations used were: mecillinam (10 μg/ml), A22 (10 μg/ml), cephalexin (10 μg/ml), and cefsulodin (100 μg/ml). Results are the average of three independent experiments with the error bars representing the standard deviation. The drop in precursor levels between untreated and mecillinam-treated WT cells is significant (p < 0.005). See text for details.