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. Author manuscript; available in PMC: 2015 Dec 4.
Published in final edited form as: Cell. 2014 Dec 4;159(6):1300–1311. doi: 10.1016/j.cell.2014.11.017

Figure 5. Effect of mecillinam on the growth and morphology of cells defective for Slt.

Figure 5

A. Cells of MG1655 [WT] or HC408 [Δslt] harboring either pSC101 (vector) or pTB63 (FtsZup) were grown overnight, diluted, and spotted on agar containing the indicated concentration of mecillinam as described for Figure 2B. B. The same cells were grown to exponential phase and diluted to an OD600 of 0.025 into LB medium containing the indicated concentration of mecillinam. Growth was continued for an additional 3 hours and the cells were fixed. The fixed cells were then imaged on agarose pads using DIC optics. Bar equals 4 microns.