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. 2014 Dec 2;14:461. doi: 10.1186/1472-6882-14-461

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© Cui et al.; licensee BioMed Central Ltd. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Effects of NNAV on TGF-β/Smad pathway in the lung tissue. Animals were treated as described in the legend to Figure 1. All rats were killed 8 weeks after bleomycin exposure. The Lung was collected, homogenized and centrifuged, and then the supernatant was collected for Western blot analysis. (A) Protein expression of TGF-β1. (B) Protein expression of p-Smad2/3. (C) Protein expression of Collagen I. (D) Protein expression of Smad7. Bars represent the mean ± SD of a minimum of N=6 per group. *P<0.05, BLM group compared to control group, #P<0.05, NNAV-treated groups compared to BLM group.