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. 2014 Nov 30;14:301. doi: 10.1186/s12866-014-0301-8

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© Leiman et al.; licensee BioMed Central Ltd. 2014

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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60-day evolved strains show diverse colony architectures, with more extreme differences visible in LB colonies. Select colony-purified evolved strains were grown from single cells on LB or from 3 μl spots on MSgg (A). For comparison, LB and MSgg colony biofilms are included from deletion mutant strains ΔsinR::spec (DS92) and ΔepsH::tet (RL3852) (B). Scale bars for LB colonies are 1 mm and for MSgg colonies are 1 cm.