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. 2014 Nov 27;7:537. doi: 10.1186/s13071-014-0537-z

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© Spindola et al.; licensee BioMed Central Ltd. 2014

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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HPLC-DAD analysis detects flavonoids as major compounds in fractions from C. australis . (A) EtOAc fraction from the leaves of C. australis showing flavonoids with RT >40 min. (B) UV spectra of flavonoids in the EtOAc fraction. (C) n-BuOH fraction from the leaves of C. australis showing flavonoids with RT <40 min. (D) UV spectra of flavonoids in the n-BuOH fraction. (E) EtOAc-Pp fraction from the leaves of C. australis showing flavonoids and tannins with 20 min < RT <50 min. (F) UV spectra of flavonoids and tannins in the EtOAc-Pp fraction.