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. 2014 Nov 27;7:520. doi: 10.1186/s13071-014-0520-8

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© Georgieva et al.; licensee BioMed Central Ltd. 2014

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Neighbour-Joining (NJ) tree for 16 species-level lineages within the ' revolutum ' group of Echinostoma based on the mitochondrial gene nad 1: Clade A. Based on a 475-nt fragment of nad1. Outgroups: Echinoparyphium aconiatum and Hypoderaeum conoideum. Numbers represent node supports from NJ and Bayesian inference (50% majority rule consensus tree) analyses (only values greater than 70 and 0.95, respectively, are shown). The newly-sequenced European isolates are shown in red; stars indicate adult isolates from natural infections. Sequence identification is as in GenBank, followed by a letter: D, Detwiler et al. [16,17]; G, Georgieva et al. [19]; K, Kostadinova et al. [28]; M, Marcilla et al. (unpublished); M & B, Morgan & Blair [14,26]. The scale-bar indicates the expected number of substitutions per site.