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In vitro Leishmania -specific cellular immune response in VL, HVL and naive groups. PBMCs were isolated and incubated with TSLA (10 μg/ml) for 120 hrs. (A-C) Cytokines (IFN-γ, TNF-α and IL-10) were measured in the supernatant of VL (n = 11), HVL (n = 16) and naive (n = 19) and (D) Granzyme B were analyzed in the supernatant of VL (n = 11), HVL (n = 12) and naive (n = 16) using CBA. Horizontal lines indicate mean values. NS, Not Significant, **p < 0.01, ***p < 0.001.
