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. Author manuscript; available in PMC: 2014 Dec 7.
Published in final edited form as: Mol Biochem Parasitol. 2008 Feb 6;159(1):44–53. doi: 10.1016/j.molbiopara.2008.01.004

Fig. 6.

Fig. 6

Expression of Giardia Nst in Saccharomyces. A. Western blot with anti-His antibodies of Saccharomyces transfected with GlNst with a C-terminal polyHis tag (lane 1) and Saccharomyces transfected with an empty vector (lane 2). B. Transport activity of closed vesicles of Saccharomyces transfected with a vector containing GlNst (black bars) or an empty vector (white bars). While transport of GDP-Man and UDP-Glc were the same, UDP-GlcNAc transport was dramatically increased in fungi transfected with GlNst. C. Plot of nucleotide-sugar transport by Saccharomyces transfected with GlNst with changing concentrations of UDP-GlcNAc (solid line). The Km of UDP-GlcNAc transport is 2.7 μM. In contrast, there was minimal or no transport of UDP-GlcNAc in Saccharomyces transfected with an empty vector (dotted line).