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. Author manuscript; available in PMC: 2015 Jun 15.
Published in final edited form as: J Immunol. 2014 Nov 10;193(12):6090–6102. doi: 10.4049/jimmunol.1401605

Figure 5. TLR2 mediated IRF7 activation requires endocytosis.

Figure 5

HEK293-TLR2 cells (A, B) or HEK293-TLR4 cells (C, D) were transfected for 24 h with the NF-κB luciferase reporter gene (A, C), or the pFR luciferase reporter gene along with plasmid expressing IRF7-Gal4 (B, D). Cells were pretreated with 100 nM Bafilomycin A or DMSO (vehicle) 1 h before stimulation with either 20 ng/ml Pam3CSK4 (A, B) or 10 ng/ml LPS (C, D). Luciferase reporter gene activity was measured 6 h later. (E) HEK293-TLR2 cells were transfected with empty vector (EV) or 10, 60 or 120 ng (wedge) of plasmid encoding TRAM WT or TRAM G2A, and the pFR luciferase reporter gene along with plasmid expressing IRF7-Gal4. Luciferase reporter gene activity was measured 30 h later. The data are mean ± SD of triplicate samples and are representative of at least three independent experiments. *p<0.05 or ***p<0.0005 compared to stimulated samples treated with DMSO (B, D) or compared to TRAM WT (E).