Table 1.

Spectral properties for 7-hydroxycoumarin derivatives

Compound	Amount (μM)	Spectral Properties
		Absorbancea	Fluorescenceb	Fluorescence
				Absorbance
7-hydroxycoumarin	50	1.48×103	1.24×106	837
7-ethoxycoumarin	50	1.30×103	9.60×104	73.8
R-7-hydroxywarfarin	10	5.64×104	4.75×106	84.2
metabolite 1d	*c	3.78×103	9.68×104	25.6
metabolite 2e	*c	1.37×105	3.69×105	2.70
S-7-hydroxywarfarin	10	5.29×104	4.48×106	84.7
metabolite 1d	*c	2.45×103	4.83×104	19.7
metabolite 2e	*c	7.89×104	2.25×105	2.84

^aArea of absorbance peak measured at 370 nm and 325 nm for coumarin and hydroxywarfarin derivatives respectively

^bArea of fluorescence emission peak measured following excitation at 370 nm and 325 nm for coumarin and hydroxywarfarin derivatives respectively

^cProduct areas observed for the reaction containing 500 μM substrate.

^dLater identified as 7-HOWAR-4-GLUC (see Discussion).

^eLater identified as 7-HOWAR-7-GLUC (see Discussion).