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. Author manuscript; available in PMC: 2015 Dec 15.
Published in final edited form as: J Immunol. 2014 Nov 5;193(12):5786–5790. doi: 10.4049/jimmunol.1401185

Figure 4. Co-culture IL-6 production correlates with greater reduction in Treg suppression in the presence of Pam3CSK4.

Figure 4

(A) The correlation (Spearman’s) between IL-6 production at 48 hours in autologous co-culture supernatants and percent reduction of suppression from the corresponding assays was performed. (B) Treg and Teff were incubated ± STAT3i (Stattic V), an inhibitor of STAT3 phosphorylation, at 1200ng/ml for one hour, then washed and co-cultured in a Treg suppression assay at a ratio of 1:2 in the presence of anti-CD3/anti-CD28 beads and media or Pam3CSK4. ‘%Reduction in suppression’ corresponds to the difference in % suppression between co-cultures treated with Pam3CSK4 or media alone expressed as a percent of media alone. The Pam3CSK4 + STAT3i culture is normalized to a media + STAT3i treated culture. Data shown are from 6 subjects, 3 of each genotype. The Pam3CSK4-induced reduction of suppression is blocked in the presence of inhibition of STAT3 phosphorylation (**p<0.01).