Table 1.
Validated gene targets whose knockdown by at least two independent siRNAs significantly enhanced ADCC by specific lysis (Fig. 2A) and/or differential cytotoxicity (Supplementary Fig. 2B) in secondary screens. siRNA target sequences can be found in Supplementary Table 2.
| Gene target | Specific lysis, p<0.05 | Differential cytotoxicity, p<0.05 |
|---|---|---|
| GRB7 | siGRB7_7, siGRB7_3 | siGRB7_7, siGRB7_3 |
| PRKCE | siPRKCE_5, siPRKCE_2 | siPRKCE_5 |
| ABL1 | siABL1_9 (“siABL1 #1”)*, | – |
| siABL1_10 (“siABL1 #2”)* |
*
,used in subsequent characterization studies (Fig. 3C)