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. Author manuscript; available in PMC: 2015 Dec 12.
Published in final edited form as: J Mol Biol. 2014 Oct 13;426(24):4049–4060. doi: 10.1016/j.jmb.2014.10.004

Fig. 7.

Fig. 7

Phosphorylation of the UbL domain in human hHR23B inhibits interaction with the proteasome. Thr-75 in hHR23B was converted to either alanine (T75A) or aspartic acid (T75D), and the protein derivatives were expressed with a Myc epitope in human lung carcinoma cell line A549. A549 contained a hHR23B-specific lentiviral expressed knock-down construct. Protein extracts were incubated with anti-Myc antibody to immunoprecipitate Myc-hHR23B and mutant derivatives. The co-precipitated proteasome was determined by immunoblot analysis using antibody against Rpn2.