Figure 1.
(A) Schematic of the BRAFV600 assay; (B) Standard curve of the BRAF assay. The equation representative of the best fit line for the BRAFV600E (lower equation) and wild type BRAF (upper equation) are shown. (C) BRAF expression level in individual cell lines including a homozygous BRAFV600E mutant line (A375), two BRAF wild-type lines (786-0, Du145), and a heterozygous line (HT29). (D) Receiver operator curve for stage IV BRAF V600E patients. With AUC of 0.9929, the assay has excellent classification ability for Stage IV melanoma.