Skip to main content
. 2014 Dec;184(12):3262–3271. doi: 10.1016/j.ajpath.2014.08.014

Figure 3.

Figure 3

FIH-1 enhances EGFR signaling to promote keratinocyte cell migration. HEKs (A) and HCEKs (B) were retrovirally transduced with either FIH-1 or empty vector (LZRS). Protein lysates from HEKs or HCEKs were harvested for immunoprecipitation (IP) or Western blot analysis (IB). Immunoprecipitated EGFR was probed for phosphotyrosine (p-Y). Radioimmunoprecipitation assay–soluble lysates were also immunoblot-analyzed for total EGFR, p-ERK, ERK, FIH-1, and GAPDH. C and D: Results from scratch-wound assays on HEKs retrovirally transduced with either FIH-1 or empty vector (LZRS) after 2-hour pretreatment with 10-μmol/L U0126 (a Mek1/2 inhibitor) and 0.1-μmol/L AG1478 (EGFR inhibitor) to inhibit the Mek1/2-ERK1/2 and EGFR pathways, respectively. Data are expressed as means ± SD (C and D). n = 3. ∗P < 0.05 versus empty vector (LZRS); †P < 0.05 versus FIH-1 with U0126 or AG1478.