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. 2014 Dec;184(12):3384–3393. doi: 10.1016/j.ajpath.2014.08.003

Figure 2.

Figure 2

Urokinase receptor (uPAR) controls the ability of 4T1 metastatic breast cancer cells to regulate expression of arginase 1 (Arg 1) in co-cultured bone marrow-derived macrophages (BMDMs). A: Flow cytometry was performed to detect mouse uPAR in 168FARN (black curve) and 4T1 (gray curve) cells and in isotype-matched IgG (dotted curve). B: Flow cytometry was performed to detect mouse uPAR in 4T1 cells transduced with a control lentiviral vector, 4T1-shCon cells (gray curve), or with a lentiviral vector encoding shRNA against uPAR, 4T1-shuPAR cells (black curve). An isotype-matched IgG control study is also shown (dotted curve). C and D: After co-culturing with cancer cells, BMDM Arg1 mRNA was analyzed by qPCR (C) and BMDM arginase activity was assessed as the arginase activity index (D). Data are expressed as means ± SEM. n = 3. ∗∗P < 0.01, ∗∗∗P < 0.001, one-way analysis of variance.