Figure 7.

IL-4 is regulated downstream of ERK1/2 in ESC cells. A: Immunoblot analysis to detect detected phosphorylated (p-) and total ERK1/2 on cell extracts from ESC1 and ESC2 cells transfected with uPAR-specific (+) or NTC siRNA (−). B: ESC1 and ESC2 cells were treated with PD98059 (50 μmol/L) or with vehicle in serum-free medium for 30 minutes. p-ERK1/2 and total ERK1/2 were determined by immunoblot analysis. C and D: ESC1 and ESC2 cells were treated with PD98059 (50 μmol/L) (white bars) or vehicle (black bars) in serum-free medium for 4 hours. mRNA levels of IL-4 (D) and TGF-β (E) were determined by quantitative real-time PCR, standardized against levels present in vehicle-treated cells. Data are expressed as means ± SEM. n = 3. ^∗P < 0.05, Student’s t-test.