Figure 3.

Histologic analysis of ureters from miR-143/145–deficient mice. Hematoxylin and eosin (A), Masson's trichrome (B), and α-SMA (C) staining of ureter sections from WT and miR-143/145 KO mice. Histologic analysis shows frequent dilatation of the proximal ureter and thinning of the smooth muscle layer in KO mice. All cell layers are present in the KO ureters. D: Quantitation of ureter smooth muscle layer thickness. Quantitation was performed in three Masson's trichrome–stained sections from each mouse. Examples of where measurements were performed are indicated by yellow bars (B). ^∗P < 0.05. WT, n = 4; KO, n = 3. Scale bars: 100 μm.