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. 2014 Dec;184(12):3226–3238. doi: 10.1016/j.ajpath.2014.08.012

Figure 6.

Figure 6

mRNA levels of genes related to smooth muscle biology in ureters from miR-143/145 mice. Expression levels of selected genes were measured by qPCR. The selection of genes was based on SPIA results. Expression levels of genes involved in vascular smooth muscle contraction (A), ECM-receptor interaction (B), and smooth muscle transcriptional regulator myocardin and its target α-SMA (C). Values are means ± SEM of four animals. Statistical analysis was performed using the Student's t-test. In most cases the differences between WT and miR-143/145 KO mice were significant. For Cacna1d, Kcnma1, Itga3, and Myocd the differences were not significant but levels were slightly higher in the mutant ureters, in agreement with the microarray results. Levels of α-SMA were not changed. Primer sequences and qPCR conditions are listed in Table 1. The expression of glyceraldehyde-3-phosphate dehydrogenase was used as an internal control. ∗P < 0.05, ∗∗P < 0.01 versus WT.